| However, whilst FT-IR spectroscopy is fast typically 10 s to 1 min per sample , the approaches for microbial sample preparation can be time consuming as plate culture or shake flasks are used for growth of the organism | A total of 95 bacteria from six different epidemiologically important multilocus sequence types ST10, ST69, ST95, ST73, ST127 and ST131 were used in this project and principal component-discriminant function analysis PC-DFA of these samples produced clear separate clustering of isolates, based on the ST |
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| coli reveal different information content in terms of metabolic potential compared to virulence factors | With this micro-culture approach we also demonstrate high reproducibility of the metabolic fingerprints |
Analysis of data using partial least squares-discriminant analysis PLS-DA , incorporating cross-validation, indicated a high prediction accuracy of 91.
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